Programmable herds help create flexible biological tools

Programmable herds help create flexible biological tools

Duke University’s biomedical engineers have developed a new platform for manufacturing biological drugs using specially designed bacteria that explode and secrete useful proteins when they feel the capsule is too crowded.

Capsules can shrink in response to changes in the bacterial population.

When it shrinks, the capsule squeezes the target protein produced by the captured bacteria.

This stand-alone platform can make it easier for researchers to create, analyze and purify a variety of biology for small-scale bio-processing.

Online research appeared on September 16 in the journal Nature Chemical Biology.

Bacteria are commonly used to produce biology, which are products such as vaccines, gene therapy and proteins that are manufactured or synthesized from biological sources.

For industrial processes, these steps are widely implemented

Although it helps produce large amounts of some molecules, this preparation is inelastic or financially viable when researchers need to produce small amounts of diverse biology or work in resource-limited environments. .

The new technology was developed by Linchong Yu, a professor of biomedical engineering at Duke University and Zhuzhun Dai, a former postdoctoral duke researcher and now an associate professor at Shenzhen Institute of Advanced Technology.

In an earlier proof of concept, you and his team e. Cooley designed a non-causative strain of bacteria when the bacteria reached a certain density.

These herds were then limited to a capsule, then bathed in antibiotics.

If the capsule bacteria were released, they were destroyed, but if they lived inside the container where the population was high, they survived.

The geometric herds can still feel their density and incarceration, but we’ve introduced a substance that can react when the number of bacteria inside them changes.

It’s as if the two components talk to each other.” Collectively they give you very dynamic behavior . ”

Once the population inside the capsule reaches a certain density, the bacteria begin to “pop” and release all their cellular contents, including protein products of interest.

At the same time, this bacterial growth alters the chemical environment inside the capsule, which causes it to shrink.

As it shrinks, it exits the released proteins from the explosive cells while bacteria and cellular feces are placed inside the capsule.

Once the protein is collected, researchers can add nutrient regeneration to the dish as a signal to expand the capsule.

This resets the internal environment and allows the bacteria to begin the regrowth process.

According to you, this cycle can be repeated for a week

To make the approach useful for bio-synthesis, the team added capsules to a microfluidic chip, which included a room for the detection and identification of proteins.

This can be replaced by a purification room so that proteins can be prepared for use in biology.

“It’s a very compact process. It doesn’t need electricity and doesn’t need a centrifuge to produce and separate these proteins.” “This makes it a good platform for bio-manufacturing.

You have the ability to produce a particular type of drug in a highly compact format at a low cost, easy to connect. At the top, this easy-to-produce platform saves the way. Multiple proteins simultaneously.”

According to Yu, this ease of use led the team to be head of Bioengineering Engineering at Duke, Ashutosh Chilcote and Alan L. Kaganov has been able to produce, measure and purify more than 50 different proteins in collaboration with the laboratory of the professor and the chairman.

They also explored how their platform could simplify the formation of protein complexes, which consist of polypeptide structures.

We were able to use seven versions of our microbial microbial boats, each of which was programmed to produce a different enzyme I said,” I said.

“Usually, to produce a metabolic pathway, you have to balance the supply chain, which may include inhibiting the expression of one enzyme and minimizing another expression. Set the right percentage for the herd.”

“This technology is incredibly versatile,” he said. “This is the capacity we want to take advantage of.”

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